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Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: FPOA induces the apoptosis of HepG2 cells
doi: 10.3892/etm.2018.5718
Figure Lengend Snippet: Western blotting analysis of protein extracts obtained from HepG2 cells treated with FPOA (0, 12.5, 25 and 50 µg/ml) for 24 h. Western blot and quantification of (A) PARP and cleaved PARP proteins, (B) Bax/Bcl-2, (C) cyt c, (D) caspase-9 and (E) caspase-3. *P<0.05, **P<0.01 vs. the control group. FPOA, 3-acetoxylanosta-8,24-dien-21-oic acid; PARP, poly (ADP) ribose polymerase; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; cyt c, cytochrome c.
Article Snippet: The membranes were then blocked with 5% (w/v) non-fat dry milk in Tris-buffered saline-Tween-20 (TBST) for 1 h at room temperature and then incubated with the primary antibodies directed against poly(ADP-ribose) polymerase (PARP; cat. no. ENM0145; 1:3,000), Bcl-2 (cat. no. ENT0470; 1:1,000), Bax (cat. no. ENT0456; 1:1,000), caspase-9 (cat. no. ESAP14070; 1:750),
Techniques: Western Blot